MED1 antibody (pThr1457)

Catalog No. ABIN7138778

Product Details anti-MED1 Antibody

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Binding Specificity: pThr1457

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity: Human, Mouse

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of Threonine 1457(A-Y-T(p)-P-Q) derived from Human PPAR-BP.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for MED1

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Alternative Name: MED1 (MED1 Products)

Background:

Background: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Aliases: Activator-recruited cofactor 205 kDa component antibody, ARC205 antibody, CRSP1 antibody, CRSP200 antibody, DRIP205 antibody, DRIP230 antibody, MED1 antibody, MED1_HUMAN antibody, Mediator complex subunit 1 antibody, Mediator of RNA polymerase II transcription subunit 1 antibody, p53 regulatory protein RB18A antibody, PBP antibody, Peroxisome proliferator-activated receptor-binding protein antibody, PPAR binding protein antibody, PPAR-binding protein antibody, PPARBP antibody, PPARGBP antibody, RB18A antibody, Thyroid hormone receptor-associated protein complex 220 kDa component antibody, Thyroid receptor-interacting protein 2 antibody, TR-interacting protein 2 antibody, Trap220 antibody, TRIP-2 antibody, TRIP2 antibody, Vitamin D receptor-interacting protein complex component DRIP205 antibody

UniProt: Q15648

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha