RASGRF1 antibody (pSer916)

Catalog No. ABIN7138730

Product Details anti-RASGRF1 Antibody

Target: RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))

Binding Specificity: pSer916

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASGRF1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Cross-Reactivity: Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of Serine 916(N-E-G(p)-T P) derived from Human Ras-GRF1.

Isotype: IgG

Application Details

Application Notes: IHC:1:50-1:100,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for RASGRF1

Target: RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))

Alternative Name: RASGRF1 (RASGRF1 Products)

Synonyms: CDC25 antibody, CDC25L antibody, GNRP antibody, GRF1 antibody, GRF55 antibody, H-GRF55 antibody, PP13187 antibody, ras-GRF1 antibody, AI844718 antibody, CDC25Mm antibody, Grf1 antibody, Grfbeta antibody, P190-A antibody, Ras-GRF1 antibody, p190 antibody, p190RhoGEF antibody, cdc25 antibody, rasgrf1 antibody, Ras protein specific guanine nucleotide releasing factor 1 antibody, RAS protein-specific guanine nucleotide-releasing factor 1 antibody, Ras protein specific guanine nucleotide releasing factor 1 S homeolog antibody, si:ch211-234p18.3 antibody, RASGRF1 antibody, Rasgrf1 antibody, rasgrf1.S antibody, si:ch211-234p18.3 antibody, rasgrf1 antibody

Background:

Background:

Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.

Huibin Yang, J. Biol. Chem., Apr 2003, 278: 13278 - 13285.
Huibin Yang, Mol. Biol. Cell, May 2006, 17: 2177 - 2189.
Raymond R. Mattingly, J. Biol. Chem., Dec 1999, 274: 37379.
Tie-Nian Zhu, J. Biol. Chem., May 2007, 282: 14816 - 14826.

Aliases: CDC25 antibody, CDC25L antibody, GNRP antibody, GRF1 antibody, GRF55 antibody, Guanine nucleotide exchange factor antibody, Guanine nucleotide releasing factor 55 kD antibody, Guanine nucleotide releasing protein antibody, guanine nucleotide-releasing factor 1 antibody, Guanine nucleotide-releasing protein antibody, H GRF55 antibody, HGRF55 antibody, PP13187 antibody, Ras protein specific guanine nucleotide releasing factor 1 antibody, Ras specific guanine nucleotide releasing factor antibody, Ras specific guanine nucleotide releasing factor CDC25 homolog antibody, Ras specific nucleotide exchange factor CDC25 antibody, Ras-GRF1 antibody, Ras-specific guanine nucleotide-releasing factor 1 antibody, Ras-specific nucleotide exchange factor CDC25 antibody, RASGRF1 antibody, RGRF1_HUMAN antibody

UniProt: Q13972

Pathways: RTK Signaling, Cell Division Cycle, M Phase