BAD antibody (pSer91, pSer128)

Catalog No. ABIN7138728

Product Details anti-BAD Antibody

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Binding Specificity: pSer91, pSer128

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAD antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of Serine 91 (E-P-S(p)-P-F) derived from Human BAD or Serine 128 (E-P-S(p)-P-F) derived from Mouse BAD.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IHC:1:50-1:100,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for BAD

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Alternative Name: BAD (BAD Products)

Synonyms: bad antibody, fa01b12 antibody, wu:fa01b12 antibody, wu:fa96d04 antibody, BAD antibody, BBC2 antibody, BCL2L8 antibody, AI325008 antibody, Bbc2 antibody, BCL2-associated agonist of cell death b antibody, BCL2 associated agonist of cell death antibody, BCL2-associated agonist of cell death antibody, badb antibody, BAD antibody, Bad antibody

Background:

Background:

The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Wang H.-G., Cell 87:629-638(1996).
Ottilie S., J. Biol. Chem. 272:30866-30872(1997).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

Aliases: AI325008 antibody, BAD antibody, BAD_HUMAN antibody, BBC 2 antibody, BBC2 antibody, BBC6 antibody, Bcl 2 Antagonist of Cell Death antibody, Bcl 2 Binding Component 6 antibody, BCL X / BCL 2 Binding Protein antibody, BCL X Binding Protein antibody, Bcl XL/Bcl 2 Associated Death Promoter antibody, Bcl-2-binding component 6 antibody, Bcl-2-like protein 8 antibody, Bcl-XL/Bcl-2-associated death promoter antibody, Bcl2 antagonist of cell death antibody, BCL2 antagonist of cell death protein antibody, BCL2 associated agonist of cell death antibody, Bcl2 Associated Death Promoter antibody, BCL2 binding component 6 antibody, BCL2 binding protein antibody, Bcl2 Like 8 Protein antibody, Bcl2-L-8 antibody, BCL2L8 antibody, Proapoptotic BH3 Only Protein antibody

UniProt: Q92934

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events