PKMYT1 antibody (pSer83)

Catalog No. ABIN7138712

This Rabbit Polyclonal antibody specifically detects PKMYT1 in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for PKMYT1 antibody (pSer83) (ABIN7138712)

Target: PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKMYT1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Product Details anti-PKMYT1 Antibody

Binding Specificity: pSer83

Purpose: Phospho-PKMYT1 (Ser83) Antibody

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of Serine 83(R-V-S(p)-F-R) derived from Human MYT1.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for PKMYT1

Target: PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))

Alternative Name: PKMYT1

Background:

Background:

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Booher R.N., J. Biol. Chem. 272:22300-22306(1997).
Liu F., Mol. Cell. Biol. 17:571-583(1997).
Nousiainen M., Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006).

Aliases: cdc2 inhibitory kinase antibody, DKFZp547K1610 antibody, FLJ20093 antibody, Membrane associated tyrosine and threonine specific cdc2 inhibitory kinase antibody, Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase antibody, MYT1 antibody, Myt1 kinase antibody, PKMYT 1 antibody, Pkmyt1 antibody, PMYT1_HUMAN antibody, PPP1R126 antibody, Protein kinase membrane associated tyrosine/threonine 1 antibody, Protein kinase Myt1 antibody, Protein phosphatase 1 regulatory subunit 126 antibody

UniProt: Q99640

Pathways: Mitotic G1-G1/S Phases, M Phase