Splicing Factor 1 antibody (pSer82)

Catalog No. ABIN7138709

Product Details anti-Splicing Factor 1 Antibody

Target: Splicing Factor 1 (SF1)

Binding Specificity: pSer82

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Splicing Factor 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity: Human, Mouse

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of Serine 82(S-P-S(p)-P-E) derived from Human SF1.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IHC:1:50-1:100,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for Splicing Factor 1

Target: Splicing Factor 1 (SF1)

Alternative Name: SF1 (SF1 Products)

Background:

Background:

Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.

Susan Y. Park, Development, May 2005, 132: 2415 - 2423.
Dujin Zhou, Mol. Endocrinol., Jul 2000, 14: 986.
Dagmar Wilhelm, Genes & Dev., Jul 2002, 16: 1839.
Philipp Gut,Development, Oct 2005, 132: 4611 - 4619.

Aliases: SF1 antibody, ZFM1 antibody, ZNF162 antibody, Splicing factor 1 antibody, Mammalian branch point-binding protein antibody, BBP antibody, mBBP antibody, Transcription factor ZFM1 antibody, Zinc finger gene in MEN1 locus antibody, Zinc finger protein 162 antibody

UniProt: Q15637

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location