14-3-3 zeta antibody (pSer58)

Catalog No. ABIN7138654

Product Details anti-14-3-3 zeta Antibody

Target: 14-3-3 zeta (YWHAZ)

Binding Specificity: pSer58

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 zeta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 58 (R-S-S(p)-W-R) derived from Human 14-3-3 zeta.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for 14-3-3 zeta

Target: 14-3-3 zeta (YWHAZ)

Alternative Name: YWHAZ (YWHAZ Products)

Background:

Background: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.

Gu YM, et al. (2006) FEBS Lett , 580(1): 305-310
Powell DW, et al. (2003) Mol Cell Biol, 23(15): 5376-5387
Mackintosh C. (2004) Biochem J, 381(Pt 2): 329-342.

Aliases: 14 3 3 delta antibody, 14 3 3 protein zeta/delta antibody, 14 3 3 protein/cytosolic phospholipase A2 antibody, 14 3 3 zeta antibody, 14-3-3 protein zeta/delta antibody, 1433Z_HUMAN antibody, Epididymis luminal protein 4 antibody, Epididymis secretory protein Li 3 antibody, HEL S 3 antibody, HEL4 antibody, KCIP-1 antibody, KCIP1 antibody, MGC111427 antibody, MGC126532 antibody, MGC138156 antibody, Phospholipase A2 antibody, Protein kinase C inhibitor protein 1 antibody, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide antibody, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta antibody, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody, Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody, YWHAD antibody, YWHAZ antibody

UniProt: P63104

Pathways: Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location