AMPK alpha antibody (pSer487)

Catalog No. ABIN7138633

Product Details anti-AMPK alpha Antibody

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Binding Specificity: pSer487

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AMPK alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 487 (S-G- SP-V-S) derived from Human AMPKa1.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for AMPK alpha

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Alternative Name: PRKAA1/PRKAA2 (SNF1A Products)

Synonyms: AMPK antibody, AMPK2 antibody, AMPKa2 antibody, PRKAA antibody, protein kinase AMP-activated catalytic subunit alpha 2 antibody, PRKAA2 antibody

Background:

Background:

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Kim JE, et al. (2005) J Proteome Res. 4(4): 1339-1346.
Woods A, et al. (2003) J Biol Chem. 278(31): 28434-28442.

Aliases: 5 AMP activated protein kinase alpha 1catalytic subunit antibody, 5 AMP activated protein kinase catalytic alpha 1 chain antibody, 5' AMP activated protein kinase catalytic subunit alpha 1 antibody, 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody, AAPK1 antibody, AAPK1_HUMAN antibody, ACACA kinase antibody, acetyl CoA carboxylase kinase antibody, AI194361 antibody, AI450832 antibody, AL024255 antibody, AMP -activate kinase alpha 1 subunit antibody, AMP-activate kinase alpha 1 subunit antibody, AMP-activated protein kinase, catalytic, alpha -1 antibody, AMPK 1 antibody, AMPK alpha 1 antibody, AMPK alpha 1 chain antibody, AMPK antibody, AMPK subunit alpha 1 antibody, AMPK subunit alpha-1 antibody, AMPK1 antibody, AMPKa1 antibody, AMPKalpha1 antibody, C130083N04Rik antibody, cb116 antibody, EC 2.7.11.1 antibody, HMG CoA reductase kinase antibody, HMGCR kinase antibody, hormone sensitive lipase kinase antibody, Hydroxymethylglutaryl CoA reductase kinase antibody, im:7154392 antibody, kinase AMPK alpha1 antibody, MGC33776 antibody, MGC57364 antibody, OTTHUMP00000161795 antibody, OTTHUMP00000161796 antibody, PRKAA 1 antibody, PRKAA1 antibody, Protein kinase AMP activated alpha 1 catalytic subunit antibody, SNF1-like protein AMPK antibody, SNF1A antibody, Tau protein kinase PRKAA1 antibody, wu:fa94c10 antibody

UniProt: Q13131

Pathways: Warburg Effect