EIF4B antibody (pSer422)
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- Target See all EIF4B Antibodies
- EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))
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Binding Specificity
- pSer422
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This EIF4B antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Cross-Reactivity
- Human, Mouse
- Purification
- Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
- Immunogen
- Peptide sequence around phosphorylation site of Serine 422 (T-G-S(p)-E-S) derived from Human eIF4B.
- Isotype
- IgG
- Top Product
- Discover our top product EIF4B Primary Antibody
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- Application Notes
- WB:1:500-1:1000, IHC:1:50-1:100,
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- Target
- EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))
- Alternative Name
- EIF4B (EIF4B Products)
- Background
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Background: Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Gingras, A.C. et al. (2001) Genes Dev. 15, 807-826.
Duncan, R. and Hershey, J.W. (1985) J. Biol. Chem. 260, 5493-5497.
Duncan, R.F. and Hershey, J.W. (1989) J. Cell Biol. 109, 1467-1481.Aliases: 2310046H11Rik antibody, AL024095 antibody, C85189 antibody, EIF 4B antibody, eIF-4B antibody, EIF4B antibody, Eukaryotic initiation factor 4B antibody, Eukaryotic translation initiation factor 4B antibody, IF4B_HUMAN antibody, PRO1843 antibody
- UniProt
- P23588
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