PKM antibody (pSer37)
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- Target See all PKM Antibodies
- PKM (Pyruvate Kinase M1/2 (PKM))
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Binding Specificity
- pSer37
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PKM antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunofluorescence (IF)
- Cross-Reactivity
- Human
- Purification
- Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
- Immunogen
- Peptide sequence around phosphorylation site of serine 37(I-D-S(p)-P-P) derived from Human PKM2.
- Isotype
- IgG
- Top Product
- Discover our top product PKM Primary Antibody
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- Application Notes
- WB:1:500-1:1000, IF:1:100-1:200,
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- Target
- PKM (Pyruvate Kinase M1/2 (PKM))
- Alternative Name
- PKM (PKM Products)
- Background
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Background:
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Aliases: PKM, PK3, OIP3, PK2
- Pathways
- Warburg Effect
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