NCF1 antibody (pSer345)

Catalog No. ABIN7138566

Product Details anti-NCF1 Antibody

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Binding Specificity: pSer345

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity: Human

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of Serine 345(P-Q-S(p)-P-G) derived from Human Nuclear p47 phox.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IHC:1:50-1:100,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Alternative Name: NCF1 (NCF1 Products)

Synonyms: NCF1A antibody, NOXO2 antibody, SH3PXD1A antibody, p47phox antibody, Ncf-1 antibody, p47 antibody, neutrophil cytosolic factor 1 antibody, NCF1 antibody, Ncf1 antibody

Background:

Background:

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Volpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989).
Lomax K.J., Science 245:409-412(1989).
Rodaway A.R.F., Mol. Cell. Biol. 10:5388-5396(1990).

Aliases: 47 kDa autosomal chronic granulomatous disease protein antibody, 47 kDa neutrophil oxidase factor antibody, NADPH oxidase organizer 2 antibody, NCF 47K antibody, NCF-1 antibody, NCF-47K antibody, Ncf1 antibody, NCF1_HUMAN antibody, Neutrophil cytosol factor 1 antibody, Neutrophil cytosolic factor 1 antibody, neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) antibody, Neutrophil NADPH oxidase factor 1 antibody, Nox organizer 2 antibody, Nox organizing protein 2 antibody, Nox-organizing protein 2 antibody, NOXO2 antibody, p47 phox antibody, p47-phox antibody, SH3 and PX domain containing protein 1A antibody, SH3 and PX domain-containing protein 1A antibody, SH3PXD1A antibody

UniProt: P14598

Pathways: PI3K-Akt Signaling