His Tag antibody
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- Target See all His Tag Antibodies
- His Tag
- Reactivity
- Please inquire
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This His Tag antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Purification
- Protein A purified
- Immunogen
- HHHHHH(6x His) synthetic peptide conjugate to KLH
- Clone
- 3G5H8
- Isotype
- IgG2a
- Top Product
- Discover our top product His Tag Primary Antibody
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- Application Notes
- Recommended dilution: WB:1:500-1:2000,
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4 - Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- Target
- His Tag
- Alternative Name
- 6*His (His Tag Products)
- Target Type
- Tag
- Background
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Background: A His tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the His codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.
Aliases: 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag
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