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His Tag antibody

WB, ELISA Host: Mouse Monoclonal 3G5H8 unconjugated
Catalog No. ABIN7131650
  • Target See all His Tag Antibodies
    His Tag
    Reactivity
    Please inquire
    Host
    • 88
    • 60
    • 11
    • 9
    • 5
    • 4
    • 1
    • 1
    Mouse
    Clonality
    • 90
    • 76
    • 10
    Monoclonal
    Conjugate
    • 76
    • 21
    • 13
    • 13
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This His Tag antibody is un-conjugated
    Application
    • 138
    • 88
    • 37
    • 25
    • 24
    • 16
    • 14
    • 14
    • 13
    • 13
    • 13
    • 7
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Purification
    Protein A purified
    Immunogen
    HHHHHH(6x His) synthetic peptide conjugate to KLH
    Clone
    3G5H8
    Isotype
    IgG2a
    Top Product
    Discover our top product His Tag Primary Antibody
  • Application Notes
    Recommended dilution: WB:1:500-1:2000,
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Preservative: 0.03 % Proclin 300
    Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C,-80 °C
    Storage Comment
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • Target
    His Tag
    Alternative Name
    6*His (His Tag Products)
    Target Type
    Tag
    Background

    Background: A His tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the His codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.

    Aliases: 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag

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