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Recombinant Topoisomerase I antibody

TOP1 Reactivity: Human WB, ELISA, IHC, IP, FACS Host: Rabbit Monoclonal 6D8 unconjugated Recombinant Antibody
Catalog No. ABIN7127850
  • Target See all Topoisomerase I (TOP1) Antibodies
    Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 63
    • 25
    • 15
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 50
    • 11
    • 2
    Rabbit
    Clonality
    • 42
    • 21
    Monoclonal
    Conjugate
    • 36
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This Topoisomerase I antibody is un-conjugated
    Application
    • 39
    • 17
    • 16
    • 12
    • 10
    • 7
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS)
    Cross-Reactivity
    Human
    Purification
    Affinity-chromatography
    Immunogen
    A synthesized peptide derived from human TOP1
    Clone
    6D8
    Isotype
    IgG
    Top Product
    Discover our top product TOP1 Primary Antibody
  • Application Notes
    Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, FC:1:20-1:200, IP:1:200-1:1000,
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C,-80 °C
    Storage Comment
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • Target
    Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))
    Alternative Name
    TOP1 (TOP1 Products)
    Target Type
    Viral Protein
    Background

    Background: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

    Aliases: DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I), TOP1

    UniProt
    P11387
    Pathways
    Caspase Cascade in Apoptosis, Stem Cell Maintenance
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