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Recombinant CDK2 antibody (pTyr15)

CDK2 Reactivity: Human WB, IHC, ELISA, IP Host: Rabbit Monoclonal 2C4 unconjugated Recombinant Antibody
Catalog No. ABIN7127684
  • Target See all CDK2 Antibodies
    CDK2 (Cyclin-Dependent Kinase 2 (CDK2))
    Antibody Type
    Recombinant Antibody
    Binding Specificity
    • 28
    • 27
    • 19
    • 16
    • 15
    • 9
    • 9
    • 8
    • 8
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr15
    Reactivity
    • 184
    • 103
    • 85
    • 11
    • 10
    • 8
    • 8
    • 7
    • 7
    • 6
    • 6
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    Human
    Host
    • 187
    • 19
    • 1
    Rabbit
    Clonality
    • 179
    • 28
    Monoclonal
    Conjugate
    • 97
    • 17
    • 15
    • 10
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This CDK2 antibody is un-conjugated
    Application
    • 169
    • 75
    • 52
    • 50
    • 47
    • 39
    • 27
    • 21
    • 20
    • 12
    • 10
    • 8
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)
    Cross-Reactivity
    Human
    Purification
    Affinity-chromatography
    Immunogen
    A synthesized peptide derived from human Phospho-CDK2 (Y15)
    Clone
    2C4
    Isotype
    IgG
    Top Product
    Discover our top product CDK2 Primary Antibody
  • Application Notes
    Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000,
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C,-80 °C
    Storage Comment
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • Target
    CDK2 (Cyclin-Dependent Kinase 2 (CDK2))
    Alternative Name
    CDK2 (CDK2 Products)
    Synonyms
    p33(CDK2) antibody, A630093N05Rik antibody, wu:fa10c02 antibody, zgc:56598 antibody, zgc:77684 antibody, eg1 antibody, CDKN2 antibody, cdk2L antibody, cyclin dependent kinase 2 antibody, cyclin-dependent kinase 2 antibody, cyclin-dependent kinase 2 S homeolog antibody, CDK2 antibody, Cdk2 antibody, cdk2 antibody, cdk2.S antibody
    Background

    Background: Serine/threonine-protein kinase involved in the control of the cell cycle, essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression, controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2, activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis, regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity). Phosphorylates CDK2AP2 (PubMed:12944431).

    Aliases: Cyclin-dependent kinase 2, Cell division protein kinase 2, p33 protein kinase, CDK2, CDKN2

    UniProt
    P24941
    Pathways
    PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
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