Recombinant DGCR8 antibody
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- Target See all DGCR8 Antibodies
- DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))
- Antibody Type
- Recombinant Antibody
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This DGCR8 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Cross-Reactivity
- Human
- Purification
- Affinity-chromatography
- Immunogen
- A synthesized peptide derived from human DGCR8
- Clone
- 2H2
- Isotype
- IgG
- Top Product
- Discover our top product DGCR8 Primary Antibody
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- Application Notes
- Recommended dilution: WB:1:500-1:5000,
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- Target
- DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))
- Alternative Name
- DGCR8 (DGCR8 Products)
- Synonyms
- MGC78846 antibody, DGCR8 antibody, gy1 antibody, dgcrk6 antibody, C22orf12 antibody, DGCRK6 antibody, Gy1 antibody, pasha antibody, D16H22S1742E antibody, D16H22S788E antibody, D16Wis2 antibody, N41 antibody, Vo59c07 antibody, si:ch211-106a19.4 antibody, wu:fc23f08 antibody, wu:fc38f06 antibody, DGCR8 microprocessor complex subunit L homeolog antibody, DiGeorge syndrome critical region gene 8 antibody, DGCR8 microprocessor complex subunit antibody, DGCR8, microprocessor complex subunit antibody, microRNA 3618 antibody, dgcr8.L antibody, DGCR8 antibody, dgcr8 antibody, Dgcr8 antibody, MIR3618 antibody
- Background
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Background: Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal (By similarity).
Aliases: Microprocessor complex subunit DGCR8, DiGeorge syndrome critical region 8, DGCR8, C22orf12, DGCRK6, LP4941
- UniProt
- Q8WYQ5
- Pathways
- Regulatory RNA Pathways
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