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beta Actin antibody (AA 1-50)

Name: beta Actin antibody (AA 1-50)
Brand: antibodies-online
SKU: ABIN7127368
Price: 220 USD
Availability: InStock

ACTB Reactivity: Human WB, ELISA, IHC, IF, IP, FACS Host: Mouse Monoclonal 1D12E12B5 unconjugated

Western Blot Positive WB detected in: Rabbit liver tissue, Rabbit brain tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit stomach tissue, A549 whole cell lysate All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

IHC image of ABIN7127368 diluted at 1:500 and staining in paraffin-embedded breast cancer performed on a Leica BondTM system. (beta Actin antibody (AA 1-50))

IHC

Western Blot Positive WB detected in: Mouse liver tissue, Mouse brain tissue, Mouse lung tissue, Mouse kidney tissue, Mouse spleen tissue, Mouse stomach tissue All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

Western Blot Positive WB detected in: Rat liver tissue, Rat brain tissue, Rat lung tissue, Rat stomach tissue, U87 whole cell lysate All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

Western Blot Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, Mouse kidney tissue, Rabbit kidney tissue All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

Western Blot Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, Rat spleen tissue, Rabbit kidney tissue All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

Western Blot Positive WB detected in: 293T whole cell lysate at 40 μg, 20 μg, 10 μg All lanes:ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

Western Blot Positive WB detected in: 20 μg 293T whole cell lysate ACTB antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

IHC image of ABIN7127368 diluted at 1:500 and staining in paraffin-embedded colon cancer performed on a Leica BondTM system. (beta Actin antibody (AA 1-50))

IHC

9 images

Catalog No. ABIN7127368

Datasheet as PDF

Target See all beta Actin (ACTB) Antibodies

beta Actin (ACTB) (Actin, beta (ACTB))
Binding Specificity
40

18

15

6

6

5

5

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-50
Reactivity
192

138

120

65

52

44

37

37

32

25

21

20

12

10

9

8

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1
Human
Host
115

93

5

1

1
Mouse
Compatible Secondaries
Clonality
114

99
Monoclonal
Conjugate
129

14

13

11

4

4

4

4

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This beta Actin antibody is un-conjugated
Application
175

77

59

50

35

30

26

21

13

13

6

6

6

5

5

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Cross-Reactivity

Human, Mouse, Rabbit, Rat

Purification

Protein A purified

Immunogen

A synthesized peptide derived from human Beta-Actin (1-50AA)

Clone

1D12E12B5

Isotype

IgG2b

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ACTB Reactivity: Human WB, IF, IP Host: Mouse Monoclonal unconjugated

anti-Actin, beta (ACTB) (AA 359-368) antibody
ACTB Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

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ACTB Reactivity: Mouse, Rat, Chicken, Zebrafish (Danio rerio) WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Rat WB, IF, IHC (p), FACS, DNA Mic Host: Mouse Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) (AA 1-25) antibody
ACTB Reactivity: Human, Mouse, Rat, Pig, Cow WB, IHC (p) Host: Mouse Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
KO Validated ACTB Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Chicken, Rabbit, Pig, Cow, Hamster, Goat, Fish WB, ELISA Host: Mouse Monoclonal 2D1D10 unconjugated

Application Notes

Recommended dilution: WB: 1:5000-1:80000, IHC: 1:500-1:1000, IF: 1:50-1:200, FC: 1:100-1:300, IP: 1 µL-5 µL,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name

ACTB (ACTB Products)

Synonyms

BRWS1 antibody, PS1TP5BP1 antibody, ACTA1 antibody, Actx antibody, E430023M04Rik antibody, beta-actin antibody, ps1tp5bp1 antibody, act-2 antibody, actb antibody, B-actin antibody, LOC100174883 antibody, LOC100304816 antibody, A antibody, ACT5C antibody, Ac5C antibody, Act antibody, Act-5C antibody, Act5 antibody, Act5c antibody, Actin antibody, Actin/BAP47 antibody, Actin5C antibody, BAP47 antibody, Bap47 antibody, CG4027 antibody, Dmel\\CG4027 antibody, M32055 antibody, T11 antibody, act antibody, act 5C antibody, act5C antibody, actin antibody, actin5C antibody, anon-EST:fe2D2 antibody, beta-actin/Bap47 antibody, cyt5C antibody, l(1)G0009 antibody, l(1)G0010 antibody, l(1)G0025 antibody, l(1)G0079 antibody, l(1)G0117 antibody, l(1)G0177 antibody, l(1)G0245 antibody, l(1)G0330 antibody, l(1)G0420 antibody, l(1)G0486 antibody, Bact antibody, ACT-5 antibody, ACTB antibody, B-ACTZF antibody, actba antibody, bact antibody, bactin1 antibody, bactzf antibody, wu:fd18f05 antibody, A26C1B antibody, POTE2alpha antibody, POTEACTIN antibody, Beta-actin antibody, actin beta antibody, actin, beta antibody, cytoplasmic actin OlCA1 antibody, beta-actin antibody, Actin 5C antibody, actin gamma 1 antibody, actin, beta 1 antibody, actin, beta L homeolog antibody, beta actin antibody, POTE ankyrin domain family member F antibody, actin, beta like 2 antibody, actin, cytoplasmic 1 antibody, ACTB antibody, Actb antibody, actb antibody, LOC100174883 antibody, LOC100304816 antibody, Act5C antibody, ACTG1 antibody, actb1 antibody, actb.L antibody, LOC100136352 antibody, POTEF antibody, ACTBL2 antibody, LOC109073280 antibody

Background

ACTB,ACTB,ACTB,PS1TP5BP1,beta-ac6n,β-ac6n

UniProt

P60709

Pathways

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction