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beta Actin antibody (AA 1-50)

Name: beta Actin antibody (AA 1-50)
Brand: antibodies-online
SKU: ABIN7127368
Price: 220 USD
Availability: InStock

ACTB Reactivity: Human WB, ELISA, IHC, IF, IP, FACS Host: Mouse Monoclonal 1D12E12B5 unconjugated

Western Blot Positive WB detected in: Rabbit liver tissue, Rabbit brain tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit stomach tissue, A549 whole cell lysate All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

IHC image of ABIN7127368 diluted at 1:500 and staining in paraffin-embedded breast cancer performed on a Leica BondTM system. (beta Actin antibody (AA 1-50))

IHC

Western Blot Positive WB detected in: Mouse liver tissue, Mouse brain tissue, Mouse lung tissue, Mouse kidney tissue, Mouse spleen tissue, Mouse stomach tissue All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

Western Blot Positive WB detected in: Rat liver tissue, Rat brain tissue, Rat lung tissue, Rat stomach tissue, U87 whole cell lysate All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

Western Blot Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, Mouse kidney tissue, Rabbit kidney tissue All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

Western Blot Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, Rat spleen tissue, Rabbit kidney tissue All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

Western Blot Positive WB detected in: 293T whole cell lysate at 40 μg, 20 μg, 10 μg All lanes:ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

Western Blot Positive WB detected in: 20 μg 293T whole cell lysate ACTB antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin antibody (AA 1-50))

IHC image of ABIN7127368 diluted at 1:500 and staining in paraffin-embedded colon cancer performed on a Leica BondTM system. (beta Actin antibody (AA 1-50))

IHC

9 images

Catalog No. ABIN7127368

Datasheet as PDF

Target See all beta Actin (ACTB) Antibodies

beta Actin (ACTB) (Actin, beta (ACTB))
Binding Specificity
39

15

14

6

6

5

5

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-50
Reactivity
174

125

107

55

43

41

37

25

23

22

18

17

12

10

8

7

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1
Human
Host
113

76

5

1

1
Mouse
Compatible Secondaries
Clonality
111

83
Monoclonal
Conjugate
122

12

12

10

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This beta Actin antibody is un-conjugated
Application
163

68

50

45

35

31

25

21

13

13

8

6

6

5

5

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Cross-Reactivity

Human, Mouse, Rabbit, Rat

Purification

Protein A purified

Immunogen

A synthesized peptide derived from human Beta-Actin (1-50AA)

Clone

1D12E12B5

Isotype

IgG2b

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ACTB Reactivity: Human WB, IF, IP Host: Mouse Monoclonal unconjugated

anti-Actin, beta (ACTB) (AA 359-368) antibody
ACTB Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 2-16) antibody
ACTB Reactivity: Mouse, Rat, Chicken, Zebrafish (Danio rerio) WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Rat WB, IF, IHC (p), FACS, DNA Mic Host: Mouse Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) (AA 1-25) antibody
ACTB Reactivity: Human, Mouse, Rat, Pig, Cow WB, IHC (p) Host: Mouse Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
KO Validated ACTB Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Chicken, Rabbit, Pig, Cow, Hamster, Goat, Fish WB, ELISA Host: Mouse Monoclonal 2D1D10 unconjugated

Application Notes

Recommended dilution: WB: 1:5000-1:80000, IHC: 1:500-1:1000, IF: 1:50-1:200, FC: 1:100-1:300, IP: 1 µL-5 µL,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name

ACTB (ACTB Products)

Background

ACTB,ACTB,ACTB,PS1TP5BP1,beta-ac6n,β-ac6n

UniProt

P60709

Pathways

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction