Recombinant ACTA1/ACTA2A/ACTG2 antibody

Catalog No. ABIN7127250

Product Details anti-ACTA1/ACTA2A/ACTG2 Antibody

Target: ACTA1/ACTA2A/ACTG2 (ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACTA1/ACTA2A/ACTG2 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)

Specificity: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Cross-Reactivity (Details): Human, Rabbit and Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant protein corresponding to human muscle actin

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Western Blot (0.5-1.0 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Muscle or sarcoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ACTA1/ACTA2A/ACTG2

Target: ACTA1/ACTA2A/ACTG2 (ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth))

Abstract: ACTA1/ACTA2A/ACTG2 Products

Background: ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1,Actin, Muscle Specific (Muscle Cell Marker)
Cellular localisation: Cytoplasm.

Molecular Weight: 42kDa

Gene ID: 58, 59, 70

UniProt: P62736, P68032, P68133