Recombinant Histone H3.3 antibody

Catalog No. ABIN7127093

Product Details anti-Histone H3.3 Antibody

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Histone H3.3 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0.0 to 6.6% (median value 0.8%). Increased expression of PHH3 was significantly associated with tumor thickness (p = 0.031), presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027), but not with Clark's level of invasion. High levels of PHH3 was associated with increased mitotic count (p = 0.003) and high Ki-67 expression (p = 0.002). For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc., PHH3 mAb is helpful for tumor pathological classification and prognosis.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A synthetic peptide corresponding to (ARK-pS-TGGKAPRKQLc) of Phosphohistone H3 (phospho S10)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil, liver or breast carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Alternative Name: H3F3 (H3F3A Products)

Synonyms: H3.3A antibody, H3F3 antibody, h3f3a antibody, MGC52829 antibody, wu:fb58e10 antibody, zgc:56193 antibody, zgc:86731 antibody, H3-I antibody, H3-II antibody, H3-III antibody, H3-IV antibody, H3-V antibody, H3-VI antibody, H3-VII antibody, H3-VIII antibody, H3F3A antibody, h3.3 antibody, h3.3a antibody, CG5825 antibody, Dmel\\CG5825 antibody, H3.3 antibody, His-3.3A antibody, His3.3 antibody, PH3 antibody, dH3.3A antibody, H3 histone family member 3A antibody, H3 histone, family 3A antibody, H3 histone, family 3A L homeolog antibody, H3 histone, family 3C antibody, histone H3.3 antibody, histone cluster 1, H3a antibody, Histone H3.3A antibody, H3F3A antibody, h3f3a.L antibody, h3f3a antibody, H3F3C antibody, LOC475916 antibody, LOC480110 antibody, HIST1H3A antibody, LOAG_05159 antibody, His3.3A antibody, H3f3a antibody

Background: Hist1h3a, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, Histone cluster 1, H3a, Histone H3 3 pseudogene, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l,Histone H3, phosphorylated (pSer10) (Nuclear Marker)
Cellular localisation: Nucleus. Chromosome.

Molecular Weight: 15kDa

Gene ID: 8350, 132854

UniProt: P68431