UBR2 antibody

Catalog No. ABIN7126416

Product Details anti-UBR2 Antibody

Target: UBR2 (Ubiquitin Protein Ligase E3 Component N-Recognin 2 (UBR2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This UBR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: This gene contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human UBR2 protein

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for UBR2

Target: UBR2 (Ubiquitin Protein Ligase E3 Component N-Recognin 2 (UBR2))

Alternative Name: UBR2 (UBR2 Products)

Background: BA49A4.1, C6orf133, dJ242G1.1, dJ392M17.3, DKFZp686C08114, E3 ubiquitin protein ligase UBR2, KIAA0349, MGC71112, N recognin 2, RP3 392M17.3, Ubiquitin ligase E3 alpha II, Ubiquitin protein ligase E3 alpha 2, Ubiquitin protein ligase E3 alpha II, Ubiquitin protein ligase E3 component n recognin 2,UBR2 / E3 ubiquitin-protein ligase UBR2 (Transcription Factor)
Cellular localisation: Nucleus.

Molecular Weight: 200.5kDa

Gene ID: 23304, 529925

UniProt: Q8IWV8