GRAMD4 antibody

Catalog No. ABIN7126410

Product Details anti-GRAMD4 Antibody

Target: GRAMD4 (GRAM Domain Containing 4 (GRAMD4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRAMD4 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Specificity: The gene encoding GRAMD4 (GRAM domain-containing protein 4) maps to human chromosome 22, which houses over 500 genes and is the second smallest human chromosome. GRAMD4, also designated death-inducing protein (DIP), is a 578 amino acid mitochondrial membrane protein that acts as an essential mediator of the p53-independent E2F-1 death pathway, which is frequently found to be deregulated in several types of cancers. Overexpression of GRAMD4 results in a strong apoptotic response involving caspase-3 activation and cleavage of poly(ADP-ribose)-polymerase. GRAMD4 is expressed in lung and in primary lung squamous cell carcinoma (LSCC) and shows upregulation in mitochondria by E2F1 after addition of 4-hydroxytamoxifen. This evidence suggests that GRAMD4 may be a potential target for cancer therapies.

Cross-Reactivity (Details): Human. Predicted to react in Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human GRAMD4 protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: Human liver, heart or fetal brain.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for GRAMD4

Target: GRAMD4 (GRAM Domain Containing 4 (GRAMD4))

Alternative Name: GRAMD4 (GRAMD4 Products)

Synonyms: DIP antibody, dA59H18.1 antibody, dJ439F8.1 antibody, 9930016O13 antibody, dip antibody, GRAM domain containing 4 antibody, GRAM domain containing 4 S homeolog antibody, GRAMD4 antibody, Gramd4 antibody, gramd4.S antibody

Background: Death-inducing protein, GRAM domain-containing protein 4 (GRAMD4),GRAMD4 (Transcription Factor)
Cellular localisation: Mitochondrion membrane. Colocalizes with COX4I1.

Molecular Weight: 66kDa

Gene ID: 23151, 475150

UniProt: Q6IC98