Reelin antibody (AA 1796-2115)

Catalog No. ABIN7126322

Product Details anti-Reelin Antibody

Target: Reelin (RELN)

Binding Specificity: AA 1796-2115

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Reelin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Reelin (or Reln) is a large glycoprotein that is secreted by Cajal-Retzius cells in the forebrain and by granule neurons in the cerebellum. Reelin was shown to be mutated in reeler mice, a mutation that is associated with widespread disruption of laminated regions of the brain, leading to impaired motor coordination, tremors and ataxia. Reelin protein expression is complex and changes throughout development. Reelin appears to function upstream of Dab1, in a signaling pathway that controls cell positioning in the developing brain, and is also thought to be a direct effector of the neurotrophin BDNF.

Cross-Reactivity (Details): Human, Mouse and Rodent.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 1796-2115) of human RELN protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human or mouse brain or CNS.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Reelin

Target: Reelin (RELN)

Alternative Name: RELN (RELN Products)

Synonyms: LIS2 antibody, PRO1598 antibody, RL antibody, reeler antibody, rl antibody, Reelen antibody, Rl antibody, reln antibody, RELN antibody, MGC172539 antibody, reelin antibody, reelin L homeolog antibody, RELN antibody, Reln antibody, reln antibody, reln.L antibody, LOC579060 antibody

Background: LIS2, PRO1598, Reeler, Reelin, RELN, RELN_HUMAN, RL,Reelin
Cellular localisation: Cytoplasm

Molecular Weight: 387.5kDa

Gene ID: 19699, 655654

UniProt: P78509

Pathways: Synaptic Membrane