DCP2 antibody

Catalog No. ABIN7126292

Product Details anti-DCP2 Antibody

Target: DCP2 (DCP2 Decapping Enzyme Homolog (DCP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DCP2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Specificity: The major pathway of eukaryotic mRNA decay involves deadenylation-dependent decapping followed by 5' to 3' exonucleolytic degradation. Human decapping enzyme 2 (hDcp2) is an mRNA decapping enzyme which contains intrinsic decapping activity. In nonsense-mediated decay, the human decapping complex, made up of hDcp1 and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. The decapping activator complex (Lsm1p-7p) is also involved in the recruitment of the decapping complex, indicated by data showing that Lsm1p-7p enhances the co-immunoprecipitation of the complex with mRNA. Dcp2 specifically hydrolyzes methylated capped RNA to release m7GDP, thereby aiding in mRNA degradation. Both Dcp1 and Dcp2 co-localize in the cytoplasm, which is consistent with their role in mRNA decay

Cross-Reactivity (Details): Human. Predicted to react with Mouse and Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human DCP2 protein

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), ,Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: MCF7, Daudi or Jurkat cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for DCP2

Target: DCP2 (DCP2 Decapping Enzyme Homolog (DCP2))

Alternative Name: DCP2 (DCP2 Products)

Synonyms: DDBDRAFT_0185453 antibody, DDBDRAFT_0252811 antibody, DDB_0185453 antibody, DDB_0252811 antibody, 2410015D23Rik antibody, 5730537H01Rik antibody, AL118268 antibody, NUDT20 antibody, RGD1562909 antibody, mRNA-decapping enzyme 2 antibody, hypothetical protein antibody, decapping mRNA 2 antibody, CpipJ_CPIJ019463 antibody, PTRG_05936 antibody, dcp2 antibody, MCYG_05106 antibody, MGYG_07617 antibody, PGTG_03310 antibody, Tsp_04018 antibody, DCP2 antibody, Dcp2 antibody

Background: DCP2 decapping enzyme homolog (S. cerevisiae), DFLJ33245, hDpc, m7GpppN-mRNA hydrolase, mRNA decapping enzyme 2, nudix (nucleoside diphosphate linked moiety X)-type motif 20 (NUDT20),DCP2 (Decapping mRNA 2)
Cellular localisation: Cytoplasm > P. body. Nucleus. Predominantly cytoplasmic, in processing bodies (PB). A minor amount is nuclear.

Molecular Weight: 48.4kDa

Gene ID: 167227, 443875

UniProt: Q8IU60