ECD/SGT1 antibody

Catalog No. ABIN7126219

Product Details anti-ECD/SGT1 Antibody

Target: ECD/SGT1 (ECD) (Ecdysoneless Homolog (ECD))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ECD/SGT1 antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS)

Specificity: Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes. Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins. The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing.

Cross-Reactivity (Details): Human. Predicted to react with Rabbit, Goat, Horse, Guinea pig, Dog, Pig, Rhesus monkey, Orangutan and Elephant (based on sequence homology).

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human ECD protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or 293T cells. Human testis, spleen or colon.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ECD/SGT1

Target: ECD/SGT1 (ECD) (Ecdysoneless Homolog (ECD))

Alternative Name: ECD (ECD Products)

Background: Ecdysoneless homolog, hSGT1, Human suppressor of GCR two, Protein ecdysoneless homolog, SGT1 protein,ECD / SGT1
Cellular localisation: Nucleus. Cytosol.

Molecular Weight: 72.76kDa

Gene ID: 11319, 631822

UniProt: O95905

Pathways: Regulation of Carbohydrate Metabolic Process