Follistatin antibody

Catalog No. ABIN7504142

Product Details anti-Follistatin Antibody

Target: Follistatin (FST)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Follistatin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Follistatin is a single-chain glycosylated protein that inhibits follicle stimulating hormone (FSH) release. Alternative splicing of Follistatin mRNA yields two isoforms, FS315 and FS288. FS288 is the main cell-surface form and binds to surface heparin sulphate proteoglycans. FST/4281 is a clone raised against recombinant Fst 288, and is used in combination, commonly as the capture, with antibody FST/4282 in a two site ELISA for the detection of Follistatin.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Raised to human Follistatin (Fst) 288 and recognizes both human Fst 288 and 315

Isotype: IgG1

Application Details

Application Notes:

Positive Control: Human testis or ovary.

Known Application: ELISA (Use in combination with clone FST/4282 for two-site ELISA), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for Follistatin

Target: Follistatin (FST)

Alternative Name: FST (FST Products)

Background: Follistatin, FS, FST, Active-binding protein,Follistatin / Activin Binding Protein
Cellular localisation: Secreted.

Molecular Weight: 37kDa

Gene ID: 10468, 9914

UniProt: P19883

Pathways: Negative Regulation of Hormone Secretion