Follistatin antibody

Catalog No. ABIN7504142

Product Details anti-Follistatin Antibody

Target: Follistatin (FST)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Follistatin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Follistatin is a single-chain glycosylated protein that inhibits follicle stimulating hormone (FSH) release. Alternative splicing of Follistatin mRNA yields two isoforms, FS315 and FS288. FS288 is the main cell-surface form and binds to surface heparin sulphate proteoglycans. FST/4281 is a clone raised against recombinant Fst 288, and is used in combination, commonly as the capture, with antibody FST/4282 in a two site ELISA for the detection of Follistatin.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Raised to human Follistatin (Fst) 288 and recognizes both human Fst 288 and 315

Isotype: IgG1

Application Details

Application Notes:

Positive Control: Human testis or ovary.

Known Application: ELISA (Use in combination with clone FST/4282 for two-site ELISA), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for Follistatin

Target: Follistatin (FST)

Alternative Name: FST (FST Products)

Synonyms: CG12955 antibody, CG12956 antibody, CG33466 antibody, Dmel\\CG33466 antibody, Fol1 antibody, dFS antibody, dFol1 antibody, dfs antibody, fs antibody, FS antibody, foll antibody, xfs antibody, FST antibody, fst antibody, AL033346 antibody, FOL1 antibody, Fst-288 antibody, RATFOL1 antibody, cb446 antibody, fd07h10 antibody, fst1 antibody, wu:fd07h10 antibody, Follistatin antibody, follistatin antibody, follistatin L homeolog antibody, follistatin a antibody, Fs antibody, fst antibody, FST antibody, LOC100141662 antibody, fst.L antibody, Fst antibody, fsta antibody

Background: Follistatin, FS, FST, Active-binding protein,Follistatin / Activin Binding Protein
Cellular localisation: Secreted.

Molecular Weight: 37kDa

Gene ID: 10468, 9914

UniProt: P19883

Pathways: Negative Regulation of Hormone Secretion