MICA antibody (AA 1-200)

Catalog No. ABIN7504179

Product Details anti-MICA Antibody

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Binding Specificity: AA 1-200

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MICA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: MICA and MICB are stress-induced antigens that are related to major histocompatibility complex (MHC) class I molecules. MICA and MICB are frequently expressed in epithelial tumors. These highly glycosylated cell surface proteins are stably expressed without conventional class I peptide ligands or association with i²-2-microglobulin. The expression is induced on proliferating or heat shock-stressed epithelial cells. MICA and MICB are broadly recognized by intestinal epithelial V L1 i³ L T cells expressing variable TCRs, suggesting that these antigens may play a central role in the signaling of cellular distress to evoke immune responses in the intestinal epithelium.

Cross-Reactivity (Details): Human, Mouse and Rat.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-200) of human MICA (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes:

Positive Control: HeLa or MCF-7 cells. Human breast, kidney or prostate.

Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for MICA

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Alternative Name: MICA (MICA Products)

Synonyms: MIC-A antibody, PERB11.1 antibody, MHC class I polypeptide-related sequence A antibody, MICA antibody

Background: HLA class I antigen, MHC class I chain related gene A protein antibody, MHC class I chain related protein A, MHC class I chain related protein A HLA B HLA C, MHC class I polypeptide related sequence A, MHC class I polypeptide-related sequence A, MHC class I related protein, MIC A, MIC-A, micA, PERB11.1, Stress inducible class I homolog,MICA
Cellular localisation: Cell surface. Cytoplasm.

Molecular Weight: 92kDa

Gene ID: 100507436

UniProt: Q29983

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response