PHKA1 antibody (AA 51-150)

Catalog No. ABIN708296

Product Details anti-PHKA1 Antibody

Target: PHKA1 (phosphorylase Kinase, alpha 1 (Muscle) (PHKA1))

Binding Specificity: AA 51-150

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHKA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PHKA1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PHKA1

Target: PHKA1 (phosphorylase Kinase, alpha 1 (Muscle) (PHKA1))

Alternative Name: PHKA1 (PHKA1 Products)

Synonyms: PHKA1 antibody, wu:fl37h01 antibody, 5330411D17 antibody, 9830108K24Rik antibody, Phka antibody, Pcyt1b antibody, PHKA antibody, phosphorylase kinase regulatory subunit alpha 1 antibody, phosphorylase kinase, alpha 1a (muscle) antibody, phosphorylase kinase, alpha 1 (muscle) antibody, phosphorylase kinase alpha 1 antibody, PHKA1 antibody, phka1a antibody, phka1 antibody, Phka1 antibody

Background:

Synonyms: 5330411D17, 9830108K24Rik, kinase PHKA1, KPB1, KPB1_HUMAN, MGC132604, Pcyt1b, PHKA, PHKA1, Phosphorylase b kinase regulatory subunit alpha, Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform, Phosphorylase kinase alpha M subunit, phosphorylase kinase, alpha 1 muscle, RP23 210E20.1, skeletal muscle isoform.

Background: Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D, also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1.

Gene ID: 5255

Pathways: Cellular Glucan Metabolic Process