GPR177/WLS antibody

Catalog No. ABIN7120857

Product Details anti-GPR177/WLS Antibody

Target: GPR177/WLS (WLS) (Wntless Homolog (WLS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR177/WLS antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: G protein-coupled receptor 177

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:1000, IP: 1:500-1:1000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for GPR177/WLS

Target: GPR177/WLS (WLS) (Wntless Homolog (WLS))

Alternative Name: GPR177 (WLS Products)

Synonyms: C1orf139 antibody, EVI antibody, GPR177 antibody, MRP antibody, mig-14 antibody, 5031439A09Rik antibody, AI173978 antibody, AI987742 antibody, Gpr177 antibody, gpr117 antibody, gpr177 antibody, sb:cb610 antibody, wu:fb36d04 antibody, wu:fb44d10 antibody, wu:fb50c06 antibody, zgc:64091 antibody, CG6210 antibody, Dmel\\CG6210 antibody, Evi antibody, Evi/Wls antibody, Srt antibody, Wls antibody, Wls/Evi antibody, evi antibody, srt antibody, wls/evi antibody, wntless Wnt ligand secretion mediator antibody, wntless homolog (Drosophila) antibody, wntless antibody, WLS antibody, Wls antibody, wls antibody

Background: Synonyms:C1orf139, EVI, G protein coupled receptor 177, GPR177, Protein wntless homolog, Sprinter, WLS, Wntless Background:GPR177, also known as Wntless (WLS) or Evi, is an evolutionarily conserved transmembrane protein necessary for Wnt protein secretion. GPR177 localizes to compartments of the secretory pathway, including the ER, Golgi apparatus, endosomes, and plasma membrane. GPR177 is overexpressed in several cancer types including glioma and gastric cancer. Several isoforms of GPR177 exist due to the alternative splicing, with predicted MW around 62 and 51 kDa, respectively. The sequence can be processed by removing the signal peptide, which generates a mature form of 45-46 kDa. In addition, slow migragrating band above 62 kDa can also be detected because GPR177 is modified by N-linked glycosylation (PMID: 20549736).

Molecular Weight: 46-62 kDa

Gene ID: 79971

UniProt: Q5T9L3

Pathways: WNT Signaling