POLI antibody
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- Target See all POLI Antibodies
- POLI (Polymerase (DNA Directed) iota (POLI))
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Reactivity
- Human, Rat, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This POLI antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Purification
- Immunogen affinity purified
- Purity
- ≥95 % as determined by SDS-PAGE
- Immunogen
- polymerase(DNA directed) iota
- Isotype
- IgG
- Top Product
- Discover our top product POLI Primary Antibody
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- Application Notes
- WB: 1:500-1:2000, IP: 1:500-1:2000, IHC: 1:20-1:200
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
- Expiry Date
- 12 months
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- Target
- POLI (Polymerase (DNA Directed) iota (POLI))
- Alternative Name
- POLI (POLI Products)
- Background
- Synonyms:DNA polymerase iota, Eta2, POLI, polymerase (DNA directed) iota, RAD30 homolog B, RAD30B, RAD3OB Background:Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
- Molecular Weight
- 87 kDa
- Gene ID
- 11201
- UniProt
- Q9UNA4
- Pathways
- DNA Damage Repair
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