PHAX antibody

Catalog No. ABIN7117517

Product Details anti-PHAX Antibody

Target: PHAX (phosphorylated Adaptor For RNA Export (PHAX))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHAX antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: phosphorylated adaptor for RNA export

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for PHAX

Target: PHAX (phosphorylated Adaptor For RNA Export (PHAX))

Alternative Name: PHAX (PHAX Products)

Background: Synonyms:RNUXA Background:A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex(CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP-and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs(snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner(By similarity). Plays also a role in the biogenesis of U3 small nucleolar RNA(snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated(TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA.

Molecular Weight: 55 kDa

Gene ID: 51808

UniProt: Q9H814

Pathways: Ribonucleoprotein Complex Subunit Organization