Nischarin antibody

Catalog No. ABIN7116889

Product Details anti-Nischarin Antibody

Target: Nischarin (NISCH)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nischarin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: nischarin

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for Nischarin

Target: Nischarin (NISCH)

Alternative Name: NISCH (NISCH Products)

Synonyms: NISCH antibody, IRAS antibody, 1200007D05Rik antibody, 3202002H23Rik antibody, AW494485 antibody, I-1 antibody, IR1 antibody, hIRAS antibody, nischarin antibody, nischarin S homeolog antibody, NISCH antibody, Cyan7822_6841 antibody, nisch.S antibody, nisch antibody, Nisch antibody

Background: Synonyms:IRAS, KIAA0975 Background:Acts either as the functional imidazoline-1 receptor(I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla(RVLM) neurons that exhibited rilmenidine-evoked hypotension(By similarity). Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons(By similarity). Acts as a modulator of Rac-regulated signal transduction pathways(By similarity). Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity(By similarity). Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation(By similarity). Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation(By similarity). Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells(By similarity). Inhibits lamellipodia formation, when overexpressed(By similarity). Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures.

Molecular Weight: 190-200 kDa

Gene ID: 11188

UniProt: Q9Y2I1