EIF3D antibody

Catalog No. ABIN7113953

Product Details anti-EIF3D Antibody

Target: EIF3D (Eukaryotic Translation Initiation Factor 3, Subunit D (EIF3D))

Reactivity: Human, Mouse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3D antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: eukaryotic translation initiation factor 3, subunit D

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for EIF3D

Target: EIF3D (Eukaryotic Translation Initiation Factor 3, Subunit D (EIF3D))

Alternative Name: EIF3D (EIF3D Products)

Background: Synonyms:EIF3S7 Background:Component of the eukaryotic translation initiation factor 3(eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex(43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2(PubMed:18056426).

Molecular Weight: 66 kDa

Gene ID: 8664

UniProt: O15371

Pathways: Ribonucleoprotein Complex Subunit Organization