ECD/SGT1 antibody

Catalog No. ABIN7113709

Product Details anti-ECD/SGT1 Antibody

Target: ECD/SGT1 (ECD) (Ecdysoneless Homolog (ECD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ECD/SGT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ecdysoneless homolog(Drosophila)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for ECD/SGT1

Target: ECD/SGT1 (ECD) (Ecdysoneless Homolog (ECD))

Alternative Name: ECD (ECD Products)

Background: Synonyms:ECD, GCR2, HSGT1, Protein ecdysoneless homolog, Protein SGT1, SGT1, Suppressor of GCR2 Background:Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP(PubMed:16849563, PubMed:23880345). May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes(PubMed:19919181, PubMed:9928932). Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins(PubMed:19640839). The cell cycle-regulating function may depend on its RUVBL1-mediated association with the R2TP complex(PubMed:26711270). May play a role in regulation of pre-mRNA splicing(PubMed:24722212).

Molecular Weight: 70-100 kDa

Gene ID: 11319

UniProt: O95905

Pathways: Regulation of Carbohydrate Metabolic Process