DNA Helicase B antibody

Catalog No. ABIN7113549

Product Details anti-DNA Helicase B Antibody

Target: DNA Helicase B (HELB)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNA Helicase B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: helicase(DNA) B

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for DNA Helicase B

Target: DNA Helicase B (HELB)

Alternative Name: HELB (HELB Products)

Synonyms: RGD1560103 antibody, hDHB antibody, AI447783 antibody, D10Ertd664e antibody, DNA helicase B antibody, helicase (DNA) B antibody, Helb antibody, HELB antibody

Background: Synonyms: Background:5'-3' DNA helicase involved in DNA damage response by acting as an inhibitor of DNA end resection(PubMed:25617833, PubMed:26774285). Recruitment to single-stranded DNA(ssDNA) following DNA damage leads to inhibit the nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5'-3' ssDNA translocase activity of HELB(PubMed:26774285). As cells approach S phase, DNA end resection is promoted by the nuclear export of HELB following phosphorylation(PubMed:26774285). Acts independently of TP53BP1(PubMed:26774285). Unwinds duplex DNA with 5'-3' polarity. Has single-strand DNA-dependent ATPase and DNA helicase activities. Prefers ATP and dATP as substrates(PubMed:12181327). During S phase, may facilitate cellular recovery from replication stress(PubMed:22194613).

Molecular Weight: 120-140 kDa

Gene ID: 391723

UniProt: Q8NG08