DGCR8 antibody

Catalog No. ABIN7113479

Product Details anti-DGCR8 Antibody

Target: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This DGCR8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: DiGeorge syndrome critical region gene 8

Clone: 2G8

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for DGCR8

Target: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Alternative Name: DGCR8 (DGCR8 Products)

Background: Synonyms:C22orf12, DGCRK6 Background:Component of the microprocessor complex that acts as a RNA-and heme-binding protein that is involved in the initial step of microRNA(miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts(pri-miRNAs) to release precursor miRNA(pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs(PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer(of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding(PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine(m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing(PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal(By similarity).

Molecular Weight: 120 kDa

Gene ID: 54487

UniProt: Q8WYQ5

Pathways: Regulatory RNA Pathways