NCK2 antibody (AA 301-380)

Catalog No. ABIN706331

Product Details anti-NCK2 Antibody

Target: NCK2 (NCK Adaptor Protein 2 (NCK2))

Binding Specificity: AA 301-380

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NCK2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for NCK2

Target: NCK2 (NCK Adaptor Protein 2 (NCK2))

Alternative Name: NCK2 (NCK2 Products)

Synonyms: GRB4 antibody, NCKbeta antibody, NCK2 antibody, 4833426I10Rik antibody, Grb4 antibody, Nck-2 antibody, Nck2 antibody, zgc:55283 antibody, fd20h08 antibody, im:7147817 antibody, si:ch211-147g19.3 antibody, wu:fd20h08 antibody, zgc:92024 antibody, NCK adaptor protein 2 antibody, cytoplasmic protein NCK2-like antibody, non-catalytic region of tyrosine kinase adaptor protein 2 antibody, NCK adaptor protein 2a antibody, NCK adaptor protein 2b antibody, NCK2 antibody, Nck2 antibody, LOC100227348 antibody, nck2a antibody, nck2b antibody

Background:

Synonyms: Cytoplasmic protein NCK2, NCK2, GRB4, Growth factor receptor bound protein 4, NCK adaptor protein 2, Nck-2, NCK2, NCK2_HUMAN, NCKbeta, Non-catalytic region of tyrosine kinase adaptor protein 2, Noncatalytic region of tyrosine kinase beta, SH2/SH3 adaptor protein NCK-beta, SH2/SH3 adaptor protein NCK-beta.

Background: This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].

Gene ID: 8440

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization