EGFR antibody (FITC)
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- Target See all EGFR Antibodies
- EGFR (Epidermal Growth Factor Receptor (EGFR))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This EGFR antibody is conjugated to FITC
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Application
- Flow Cytometry (FACS)
- Purpose
- Anti-EGFR FITC Antibody
- Characteristics
- The clone 528, a mouse monoclonal antibody, specifically reacts with an epitope of the ~170 kDa extracellular protein domain of human epidermal growth factor receptor or commonly known as EGFR. Physiologically EGFR is expressed in the skin, gastrointestinal system, kidney, and other normal tissues as well as aberrantly over expresses in epithelial cancer cells of lung, pancreas, colon, breast, and on the head and neck squamous cell. EGFR signaling is activated upon binding one of its ligands including epidermal growth factor (EGF), transforming growth factor α (TGF α), Amphiregulin, and heparin binding-EGF (HB-EGF). Upon activation, EGFR becomes an active homodimer from an inactive monomeric form, resulting in several downstream signal transduction cascades including the MAPK, Akt and JNK pathways, leading to DNA synthesis and cell proliferation closely linked with cancer pathogenesis. The 528 antibody has been reported to block EGF binding to its receptor and inhibits A431 tumor formation in nude mice. Therefore, the anti-EGFR monoclonal antibody based anti-cancer immunotherapy has strong clinical potential against various epithelial solid malignant tumors.
- Purification
- Purified
- Purity
- >95 %
- Grade
- GMP Grade
- Immunogen
- Purified EGFR from A431 cells
- Clone
- 528
- Isotype
- IgG2a, kappa
- Top Product
- Discover our top product EGFR Primary Antibody
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anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibody
EGFR Reactivity: Human WB, ELISA, IHC, FACS Host: Mouse Monoclonal 7A6F12 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 888-1210) antibodyEGFR Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal C4 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer1026) antibodyEGFR Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer1071) antibodyEGFR Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (pSer695) antibodyEGFR Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 1163-1191), (C-Term) antibodyEGFR Reactivity: Human WB, IHC (p) Host: Mouse Monoclonal 688CT33-1-3 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibodyEGFR Reactivity: Human WB, ELISA, FACS Host: Mouse Monoclonal 5E10D3 unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibodyEGFR Reactivity: Human, Mouse, Rat, Pig, Monkey, Cow, Chicken, Dog, Horse, Rabbit, Guinea Pig, Avian, Cat, Donkey, Goat, Hamster, Sheep WB Host: Goat Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 960-1060) antibodyEGFR Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor (EGFR) (AA 1021-1210) antibodyEGFR Reactivity: Human WB, IHC, IP Host: Rabbit Polyclonal unconjugated
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- PBS pH 7.2, 0.2 % (w/v) BSA, 0.09 % (w/v) sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- 2-8°C, Conjugated antibodies should never be frozen.
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- Target
- EGFR (Epidermal Growth Factor Receptor (EGFR))
- Alternative Name
- EGFR (EGFR Products)
- Gene ID
- 1956
- UniProt
- P00533
- Pathways
- NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins
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