Myosin Binding Subunit antibody

Catalog No. ABIN7074731

Product Details anti-Myosin Binding Subunit Antibody

Target: Myosin Binding Subunit (MBS)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myosin Binding Subunit antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human, Rat

Purification: Affinity purification

Immunogen: Recombinant protein corresponding to Mouse Myosin Phosphatase

Application Details

Application Notes: WB (H,M,R) 1: 500-1: 1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for Myosin Binding Subunit

Target: Myosin Binding Subunit (MBS)

Alternative Name: Myosin Phosphatase (MBS Products)

Background: Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.

Molecular Weight: 130 kDa

Gene ID: 17931

NCBI Accession: NP_082168

UniProt: Q9DBR7