GTF2B antibody

Catalog No. ABIN7074034

Product Details anti-GTF2B Antibody

Target: GTF2B (General Transcription Factor IIB (GTF2B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Cross-Reactivity: Human, Rat

Purification: Affinity purification

Immunogen: Recombinant protein corresponding to Mouse TFIIB

Application Details

Application Notes: WB (H,M,R) 1: 500-1: 1000, IHC/IF (H,M,R) 1:500-1:1500/1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for GTF2B

Target: GTF2B (General Transcription Factor IIB (GTF2B))

Alternative Name: TFIIB (GTF2B Products)

Background: General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA .Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex .Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle . Associates with chromatin to core promoter- specific regions .Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner,Modulates transcription start site selection . Exhibits also autoacetyltransferase activity that contributes to the activated transcription .

Molecular Weight: 35 kDa

Gene ID: 229906

NCBI Accession: NP_663521

UniProt: P62915