Recombinant CD79b antibody

Catalog No. ABIN7072739

Product Details anti-CD79b Antibody

Target: CD79b (CD79B) (CD79b Molecule, Immunoglobulin-Associated beta (CD79B))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD79b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: Anti-CD79b [HM79-16], Rabbit IgG, lambda

Specificity: Reacts with an extracellular epitope of CD79b.

Characteristics:

Original Species of Ab: Hamster, Armenian

Original Format of Ab: IgG2

Purification: Protein A affinity purified

Immunogen: HM79-16 was initially generated by purifying CD79b from WEHI231 B cells and used for immunisation of footpads of an Armenian hamster. Hybridoma supernatants were screened for positive reactivity in surface immunofluorescence of WEHI231 and then the specificity to CD79b was tested by immuno-precipitationRecombinant anti-CD79 antibodies were engineered by cloning and sequencing the full-length IG heavy and light chains of an Armenian hamster (Cricetulus migratorius).

Clone: HM79-16

Isotype: IgG lambda

Application Details

Application Notes: A biotinylated HM79-16 was used and stained with streptavidin-FITC for analysis by FACS to identify variants of B lymphoma cell lines that have lost expression of the mu heavy chain, and for staining using a biotinylated HM79-16 (Condon 2000). HM79-16 has also been used in competitive binding assays to study its effect on the binding of CD79a to CD79b in the context of ameliorating autoimmune disease in mice (Yongmei Li 2008).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Armenian Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD79b

Target: CD79b (CD79B) (CD79b Molecule, Immunoglobulin-Associated beta (CD79B))

Alternative Name: CD79b (CD79B Products)

Background: Ig-beta, AGM6, B29, IGB, CD79b molecule,

UniProt: P15530

Pathways: BCR Signaling