Recombinant CD53 antibody

Catalog No. ABIN7072633

Product Details anti-CD53 Antibody

Target: CD53

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD53 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Purpose: Anti-CD53 [161-2 (53/2)], Rabbit IgG, kappa

Specificity: This antibody recognizes human CD53. It does not react with the baboon and horse antigen. Cd53 is a member of tetraspanin family and is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunizng mice with stimulated human leukocytes.

Clone: 161-2

Isotype: IgG kappa

Application Details

Application Notes: This antibody is recommended for the detection and analysis of CD53 via flow cytometry and immunofluorescence. Additionally, one group utilized this antibody for the ligation of the CD53 antigen while researching the potential involvement of tetraspanins (such as CD53) in tumor metastasis (Yunta et al., 2002). Furthermore, CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells, as it is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD53

Target: CD53

Alternative Name: CD53 (CD53 Products)

Background: Tetraspanin-25, Leukocyte surface antigen CD53, Cell surface glycoprotein CD53, Tspan-25

UniProt: P19397