Recombinant IL-10RA antibody (Extracellular Domain)

Catalog No. ABIN7072606

Product Details anti-IL-10RA Antibody

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Antibody Type: Recombinant Antibody

Binding Specificity: Extracellular Domain

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This IL-10RA antibody is un-conjugated

Application: Flow Cytometry (FACS), Blocking Peptide (BP)

Purpose: Anti-IL-10R [1B1.3a], Rabbit IgG, kappa

Specificity: This antibody recognises mouse IL-10R which is a cell surface glycoprotein and a receprot for anti-inflammatory IL-10. The epitope recognised by this antibody has been localised to the ligand binding domain within the extracellular region of mouse IL-10R.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising rats with the purified recombinant extracellular region of mouse Interleukin 10 Receptor.

Clone: 1B1-3a

Isotype: IgG kappa

Application Details

Application Notes: 1B1.3a has been widely applied in flow cytometric analyses where the detection of IL-10R was needed. Furthermore, this antibody was also successfully utilised as a blocking agent, one group used it to demonstrate that IL-10 mediates suppressive activity, by targeting its receptor IL-10R with this antibody (Wang et al., 2004). Castro et al. (2000) demonstrated that 1B1.3a is able to make a soluble peptide or protein antigen immunogenic for subsequent Th1 recall responses in a situation when there is an additional signal, such as LPS, activating the immune system. Consequently, this antibody is a hugely promising agent in the potential development of vaccines for immunotherapy. Similarly, Vicari and colleagues (2002) showed that this antibody has an enormous potential as a therapeutic anti-tumour agent when combined with the administration of CpG to stimulate the immune system.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for IL-10RA

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Alternative Name: IL-10R (IL10RA Products)

Background: CD210, Interleukin-10 receptor subunit, Interleukin-10 receptor subunit alpha, Il10ra Il10r, IL-10 receptor subunit alpha, IL-10R subunit alpha, IL-10RA, CDw210a, Interleukin-10 receptor subunit 1, IL-10R subunit 1, IL-10R1, IBI-3A, 1B1-3A

UniProt: Q61727

Pathways: Growth Factor Binding