Recombinant CD53 antibody

Catalog No. ABIN7072601

Product Details anti-CD53 Antibody

Target: CD53

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD53 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Purpose: Anti-CD53 [HD77], Rabbit IgG, kappa

Specificity: This antibody is specific for the extracytoplasmic part of human CD53, a pan-leukocyte antigen.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising mice with human leukemia cells.

Clone: HD77

Isotype: IgG kappa

Application Details

Application Notes: This antibody has applications in the detection of normal and neoplastic white blood cells. This antibody has been used to stain tumour and mononuclear cells for CD53 expression in flow cytometric analysis of samples from ovarian carcinoma patients (Marme et al, 2002), and cells of the colon carcinoma line HT29 (Koretz et al, 1992). In immunohistochemical analysis of frozen tissue sections, this antibody has been shown to react with osteoclasts and osteoclast-like giant cells in malignant fibrous histiocytoma and in benign giant cell tumor of tendon sheath (Mechtersheimer et al, 1990). This antibody has been used to re-immunoprecipitate CD53 from PKCα immunoprecipiates (Zhang et al, 2001).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD53

Target: CD53

Alternative Name: CD53 (CD53 Products)

Background: TSPAN-25, Leukocyte surface antigen CD53, Tetraspanin-25, MOX44, TSPAN25

UniProt: P19397