Recombinant L1CAM antibody

Catalog No. ABIN7072598

Product Details anti-L1CAM Antibody

Target: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This L1CAM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Purpose: Anti-CD171 [L1-14.10], Mouse IgG1, kappa

Specificity: This antibody is specific for the ectodomain (amino acids M1 to E1123) of human L1, and recognises an epitope in the third Ig domain. L1 is over-expressed in a range of tumours, including neuroblastomas, renal carcinomas, ovarian and endometrial carcinomas, and melanomas. This antibody does not cross-react with human CHL1.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising mice with recombinant L1-Fc fusion protein, consisting of the ectodomain of human L1.

Clone: L1-14-10

Isotype: IgG1 kappa

Application Details

Application Notes: The specificity of this antibody has been confirmed in flow cytometric (Schafer et al, 2012, Huszar et al, 2006) and Western blot analysis (Huszar et al, 2006, Wolterink et al, 2010). This antibody has been shown, when incubated with SKOV3ip human ovarian carcinoma cells, to efficiently and sustainedly block extracellular signal-regulated kinase (Erk) phosphorylation (Gast et al, 2008). This antibody has been used in immunohistochemical staining for L1 expression, using paraffin-embedded human ovarian carcinoma tissue sections (Wolterink et al, 2010, Knogler et al, 2007) and a panel of human tumour tissue sections, including benign and malignant tumours of the female genital tract (Huszar et al, 2006), and renal cell carcinoma, schwannoma, gastric conventional GIST, pancreatic neuroendocrine tumor and paraganglioma (Inaguma et al, 2016). This antibody, when administered to SCID mice in combination with cytostatic drugs, efficiently reduces the growth of subcutaneously grown Colo357 or SKOV3ip tumors (Schafer et al, 2012).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for L1CAM

Target: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Alternative Name: CD171 (L1CAM Products)

Background: L1CAM, 14.10, L1, Neural cell adhesion molecule L1, N-CAM-L1, NCAM-L1

UniProt: P32004

Pathways: Synaptic Membrane