Recombinant MARCO antibody

Catalog No. ABIN7072581

Product Details anti-MARCO Antibody

Target: MARCO (Macrophage Receptor with Collagenous Structure (MARCO))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This MARCO antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Anti-MARCO [PLK1], Rabbit IgG, kappa

Specificity: This antibody recognises the extracellular domain V between residues 420 and 431 in MARCO both in human and bovine samples.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG3

Purification: Protein A affinity purified

Immunogen: The antibody was raised by immunising BALB/c mice with human alveolar macrophages (AMs) and subsequent creation of hybridomas.

Clone: PLK1

Isotype: IgG kappa

Application Details

Application Notes: The specificity of this antibody was confirmed in an IF experiment where the antibody stained alveolar macrophages for MACRO (Arredouani et al., 2005). Tissue samples prepared in acetone and paraformaldehyde were also successfully immunohistochemically stained (Arredouani et al., 2005, Martens et al., 2006). The antibody was also capable of weak staining of alveolar macrophages in flow cytometry (Palecanda and Kobzik, 2001). In IP experiments, the antibody immunoprecipitates MARCO from lysates obtained from COS cells transfected with human MARCO (Arredouani et al., 2005).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for MARCO

Target: MARCO (Macrophage Receptor with Collagenous Structure (MARCO))

Alternative Name: MARCO (MARCO Products)

Background: SCARA2, macrophage receptor MARCO, macrophage receptor with collagenous structure, scavenger receptor class A member 2, PLK-1, PLK 1

UniProt: Q9UEW3

Pathways: Activation of Innate immune Response