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Recombinant LAG3 antibody

LAG3 Reactivity: Mouse FACS, IP, BP Host: Rabbit Chimeric C9B7W unconjugated Recombinant Antibody
Catalog No. ABIN7072564
  • Target See all LAG3 Antibodies
    LAG3 (Lymphocyte-Activation Gene 3 (LAG3))
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 93
    • 34
    • 9
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Mouse
    Host
    • 53
    • 35
    • 8
    • 4
    • 4
    Rabbit
    Clonality
    • 53
    • 49
    • 1
    Chimeric
    Conjugate
    • 59
    • 6
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This LAG3 antibody is un-conjugated
    Application
    • 70
    • 43
    • 35
    • 30
    • 24
    • 22
    • 15
    • 14
    • 13
    • 12
    • 10
    • 9
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Peptide (BP)
    Purpose
    Anti-LAG-3 [C9B7W], Rabbit IgG, kappa
    Specificity
    This antibody is specific for an epitope in the D2 domain of murine LAG-3.
    Characteristics

    Original Species of Ab: Rat

    Original Format of Ab: IgG1

    Purification
    Protein A affinity purified
    Immunogen
    This antibody was raised by immunising Lewis rats with a murine LAG-3:Ig fusion protein.
    Clone
    C9B7W
    Isotype
    IgG kappa
    Top Product
    Discover our top product LAG3 Primary Antibody
  • Application Notes
    This antibody has been used in flow cytometric analysis of splenocytes (Workman et al, 2005), T cell hybridomas expressing wild-type or chimeric CD4:murine LAG-3 (Workman et al, 2002) and CD4+ regulatory T cells (Chien et al, 2017). It has also been used in immunoprecipitation analysis of murine T hybridoma 3A9 cells transduced with murine LAG-3 (Li et al, 2004). This antibody blocks LAG-3 function in vitro, inhibiting antigen-induced IL-2 production in LAG-3-expressing T cell hybridomas, but does not block LAG-3:MHC class II interaction (Workman et al, 2002). Treatment with this antibody also blocks LAG-3 function in vivo, leading to accelerated homeostatic expansion of WT T cells (Workman et al, 2005). Administration of this LAG-3-blocking antibody, in combination with PD-L1 blockade, synergistically improves the survival of lymphodepleted myeloma-bearing mice (Jing et al, 2015).
    Comment

    This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    PBS with 0.02 % Proclin 300.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • Target
    LAG3 (Lymphocyte-Activation Gene 3 (LAG3))
    Alternative Name
    Lag3 (LAG3 Products)
    Background
    CD223, LAG3, Lymphocyte activation gene 3 protein
    UniProt
    Q61790
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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