Recombinant TIGIT antibody

Catalog No. ABIN7072547

Product Details anti-TIGIT Antibody

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This TIGIT antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Blocking Peptide (BP)

Purpose: Anti-TIGIT [1B4], Rabbit IgG, lambda

Specificity: This antibody is specific for murine TIGIT.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising TIGIT-/- mice with recombinant murine TIGIT tetramers.

Clone: 1B4

Isotype: IgG lambda

Application Details

Application Notes: The binding specificity of this antibody for TIGIT has been confirmed in ELISA analysis and by flow cytometry using P815 cells transfected with mouse TIGIT and activated mouse splenocytes (Dixon et al, 2018). This antibody also specifically stains activated primary wild-type T cells (Dixon et al, 2018). This antibody fully blocks CD155 binding to TIGIT, and acts as a TIGIT blocking antibody in vivo. Administration of this antibody increases experimental autoimmune encephalomyelitis (EAE) severity, and retards the growth of established MC38 colon carcinoma and GL261 glioblastoma (Dixon et al, 2018). This antibody synergises with anti-PD-1 blocking antibodies to achieve complete tumour regression in colon carcinoma models (Dixon et al, 2018). The original mouse IgG1 antibody does not deplete TIGIT-expressing CD4+ or CD8+ T cells in vivo, nor affect T cell proliferation in vitro (Dixon et al, 2018).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for TIGIT

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Alternative Name: TIGIT (TIGIT Products)

Background: VSTM3, T cell Ig and ITIM domain, T cell immunoreceptor with Ig and ITIM domains, V-set and transmembrane domain-containing protein 3, WUCAM

UniProt: P86176

Pathways: Cancer Immune Checkpoints