Recombinant RAGE antibody
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- Target See all RAGE (AGER) Antibodies
- RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))
- Antibody Type
- Recombinant Antibody
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Chimeric
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Conjugate
- This RAGE antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Surface Plasmon Resonance (SPR)
- Purpose
- Anti-RAGE [2A11], Rabbit IgG, kappa
- Specificity
- 2A11 is specific for the V-domain of human advanced glycosylation end product-specific receptor. It recognises both the glycosylated and non-glycosylated forms.
- Characteristics
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
- Purification
- Protein A affinity purified
- Immunogen
- Human RAGE protein.
- Clone
- 2A11
- Isotype
- IgG kappa
- Top Product
- Discover our top product AGER Primary Antibody
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anti-Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 180-402) antibody
AGER Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 1-404) antibodyAGER Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 91-274) antibodyAGER Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 151-250) antibodyAGER Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IP, IF (cc), IF (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 23-404) antibodyAGER Reactivity: Human WB, ELISA, IP Host: Mouse Monoclonal 1C1 unconjugated
anti-Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 91-120), (N-Term) antibodyAGER Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 76-104) antibodyAGER Reactivity: Human, Mouse WB, ELISA Host: Rabbit Polyclonal unconjugated
anti-Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 206-234) antibodyAGER Reactivity: Human WB, IF Host: Rabbit Polyclonal RB21217 unconjugated
anti-Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 348-378), (C-Term) antibodyAGER Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB4827 unconjugated
anti-Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 24-52), (N-Term) antibodyAGER Reactivity: Human, Mouse WB, IHC (p), FACS Host: Rabbit Polyclonal RB25948 unconjugated
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- Application Notes
- ELISA confirmed the specificity of 2A11 for the V-domain of RAGE, with a binding affinity (Kd) of 7.6 nM. ELISA was also used to confirm that the binding of 2A11 to RAGE is glycosylation independent. In SPR experiments the Kd of 2A11 binding to RAGE was determined as 0.38 nM. Flow cytometry and immunochemistry were used to visualise the binding of 2A11 to HEK-RAGE cells.
- Comment
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- PBS with 0.02 % Proclin 300.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Target
- RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))
- Alternative Name
- RAGE (AGER Products)
- Background
- AGER, advanced glycosylation end product-specific receptor, receptor for advanced glycosylation end products
- Pathways
- Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins
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