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Recombinant TNFSF8 antibody

TNFSF8 Reactivity: Mouse FACS, BP, Func Host: Rat Monoclonal RM153 unconjugated Recombinant Antibody
Catalog No. ABIN7072510
  • Target See all TNFSF8 Antibodies
    TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 34
    • 24
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 36
    • 7
    • 4
    Rat
    Clonality
    • 36
    • 10
    • 1
    Monoclonal
    Conjugate
    • 30
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TNFSF8 antibody is un-conjugated
    Application
    • 31
    • 14
    • 11
    • 10
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Blocking Peptide (BP), Functional Studies (Func)
    Purpose
    Anti-CD153 [RM153], Rat IgG2b, kappa
    Specificity
    This antibody is specific for murine CD153, a 40- kDa type II transmembrane protein member of the TNF superfamily. Interaction of CD30 with CD153 plays an important role in T cell activation/costimulation.
    Characteristics

    Original Species of Ab: Rat

    Original Format of Ab: IgG2b

    Purification
    Protein A affinity purified
    Immunogen
    This antibody was raised by immunising Sprague Dawley rats with the CHO cells stably transfected with murine CD153 and fusing the splenocytes with P3U1 myeloma cells.
    Clone
    RM153
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product TNFSF8 Primary Antibody
  • Application Notes
    This antibody has been used in various FACS analyses, for instance, to demonstrate that lymphoid tissue inducer-like cells are an innate source of IL-17 and IL-22 (Takatori et al, 2009), and to suggest that CD30 ligand/CD30 plays a critical role in Th17 differentiation in mice (Sun et al, 2010). Importantly, the pharmacological effects of this rat anti-mouse CD153 mAb (clone RM153, IgG2b isotype) have been reported in a number of settings. For example, this antibody has been shown to prevent or delay the spontaneous development of diabetes in non-obese diabetic mice when treatment is initiated in young mice (Chakrabarty et al, 2003), and to prolong the survival of mice in a model of CD4+ T-cell-mediated lethal graft-versus-host disease induced by injection of C57BL/6 cells into MHC class II-only disparate bm12 recipients (Blazar et al, 2004). This antibody has also been used as parts of in vivo functional assays to demonstrate that early CD30 signaling is critical for Treg-mediated acute graft-versus-host disease protection after major MHC-mismatch bone marrow transplantation (Zeiser et al, 2007), and to provide direct evidence that pathogenic memory T cells are amenable to suppression in an antigen-specific manner while identifying CD30 as a molecule that is critical for the regulation of memory T cell responses (Dai et al, 2004).
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    PBS with 0.02 % Proclin 300.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • Target
    TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))
    Alternative Name
    CD153 (TNFSF8 Products)
    Background
    CD30-L, CD30 ligand, Tumor necrosis factor ligand superfamily member 8.
    UniProt
    P32972
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