Recombinant TNFSF8 antibody

Catalog No. ABIN7072509

Product Details anti-TNFSF8 Antibody

Target: TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This TNFSF8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Blocking Peptide (BP), Functional Studies (Func)

Purpose: Anti-CD153 [RM153], Rabbit IgG, kappa

Specificity: This antibody is specific for murine CD153, a 40- kDa type II transmembrane protein member of the TNF superfamily. Interaction of CD30 with CD153 plays an important role in T cell activation/costimulation.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising Sprague Dawley rats with the CHO cells stably transfected with murine CD153 and fusing the splenocytes with P3U1 myeloma cells.

Clone: RM153

Isotype: IgG kappa

Application Details

Application Notes: This antibody has been used in various FACS analyses, for instance, to demonstrate that lymphoid tissue inducer-like cells are an innate source of IL-17 and IL-22 (Takatori et al, 2009), and to suggest that CD30 ligand/CD30 plays a critical role in Th17 differentiation in mice (Sun et al, 2010). Importantly, the pharmacological effects of this rat anti-mouse CD153 mAb (clone RM153, IgG2b isotype) have been reported in a number of settings. For example, this antibody has been shown to prevent or delay the spontaneous development of diabetes in non-obese diabetic mice when treatment is initiated in young mice (Chakrabarty et al, 2003), and to prolong the survival of mice in a model of CD4+ T-cell-mediated lethal graft-versus-host disease induced by injection of C57BL/6 cells into MHC class II-only disparate bm12 recipients (Blazar et al, 2004). This antibody has also been used as parts of in vivo functional assays to demonstrate that early CD30 signaling is critical for Treg-mediated acute graft-versus-host disease protection after major MHC-mismatch bone marrow transplantation (Zeiser et al, 2007), and to provide direct evidence that pathogenic memory T cells are amenable to suppression in an antigen-specific manner while identifying CD30 as a molecule that is critical for the regulation of memory T cell responses (Dai et al, 2004).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for TNFSF8

Target: TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))

Alternative Name: CD153 (TNFSF8 Products)

Background: CD30-L, CD30 ligand, Tumor necrosis factor ligand superfamily member 8.

UniProt: P32972