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Recombinant Galectin 9 antibody

LGALS9 Reactivity: Mouse FACS, IP, Func, BP Host: Rabbit Chimeric RG9-35 unconjugated Recombinant Antibody
Catalog No. ABIN7072507
  • Target See all Galectin 9 (LGALS9) Antibodies
    Galectin 9 (LGALS9) (Lectin, Galactoside-Binding, Soluble, 9 (LGALS9))
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 66
    • 46
    • 19
    • 6
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Mouse
    Host
    • 82
    • 12
    • 2
    Rabbit
    Clonality
    • 80
    • 15
    • 1
    Chimeric
    Conjugate
    • 50
    • 16
    • 8
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Galectin 9 antibody is un-conjugated
    Application
    • 75
    • 34
    • 34
    • 27
    • 14
    • 14
    • 12
    • 9
    • 8
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func), Blocking Peptide (BP)
    Purpose
    Anti-Galectin 9 [RG9-35], Rabbit IgG, kappa
    Specificity
    This antibody is specific for murine galectin-9, a 40 kDa S-type lectin that is expressed by various cell types, including lymphocytes, thymocytes, macrophages, dendritic cells, astrocytes, mast cells, eosinophils, fibroblasts, epithelial cells, and endothelial cells. Galectin-9 binds to β-galactosides and can serve as a ligand for TIM-3 (CD366). The protein is implicated in both innate and adaptive immune responses, specifically, induction of cytokine secretion by macrophages, bactericidal functions, promotion of dendritic cell maturation, regulatory T cell expansion, and negative regulation of Th1, Th17, NK, and cytotoxic T cells.
    Characteristics

    Original Species of Ab: Rat

    Original Format of Ab: IgG2a

    Purification
    Protein A affinity purified
    Immunogen
    This antibody was raised by immunising rats with recombinant mouse galectin-9.
    Clone
    RG9-35
    Isotype
    IgG kappa
    Top Product
    Discover our top product LGALS9 Primary Antibody
  • Application Notes
    When this antibody was first generated and characterised, it was shown to bind with galectin-9 (Gal-9) but not with either galectin-1 or galectin-3. Furthermore, it was shown to be capable of blocking Tim-3-Fc binding to Gal-9, and inhibiting Gal-9-induced Th1 cell death in vitro (Fukushima et al., 2008, PMID: 18087160). This antibody has been used in FACS analyses. For instance, to describe a novel mechanism where Tim-3 binds HMGB-1 on virus-specific CD8+ Treg cells, suppressing proliferation of CD8+ T effector cells during acute adenoviral infection (Dolina et al., 2014, PMID: 24677194), and to indicate a crucial role for Tim-3 in phagocytosis of apoptotic cells and cross-presentation (Nakayama et al., 2009, PMID: 19224762). In addition, this antibody has been used in vivo to study the roles of Gal-9 in the development of experimental allergic conjunctivitis in mice (Fukushima et al., 2008, PMID: 18087160) and to demonstrate that constitutive expression of Gal-9 plays an immunosuppressive role in corneal allografts (Shimmura-Tomita et al., 2013, PMID: 23667648). This antibody was used in a cold immunoprecipitation (IP) assay to explore the binding of Gal-Nab1 and Gal-Nab2 to native murine gal-9, using a protein extract from murine thymus as the source of native Gal-9, RG9-35, was one of the antibodies used to isolate immune complexes formed between primary antibodies and proteins extracted from a mouse thymus for further analysis (Lhuillier et al., 2018, PMID: 30204750).
    Comment

    This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    PBS with 0.02 % Proclin 300.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • Target
    Galectin 9 (LGALS9) (Lectin, Galactoside-Binding, Soluble, 9 (LGALS9))
    Alternative Name
    Galectin 9 (LGALS9 Products)
    Background
    Ecalectin, Gal-9, galectin 9, Galectin-9, HUAT, lectin, galactose binding, soluble 5, LGALS9A, tumor antigen HOM-HD-21, urate transporter/channel protein.
    UniProt
    O08573
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