Recombinant TIMD4 antibody

Catalog No. ABIN7072500

Product Details anti-TIMD4 Antibody

Target: TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TIMD4 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), In vivo Studies (in vivo)

Purpose: Anti-Tim-4 [5G3], Rat IgG1, kappa

Specificity: This antibody is specific for murine TIM-4. TIM-4, unlike other TIM family members, is not expressed on T lymphocytes but rather found on antigen-presenting cells such as lymphoid and myeloid dendritic cells and macrophages. TIM-4 has been shown to interact with TIM-1, thereby suggesting a role of TIM-4 in the balance of Th1/Th2 regulation. Additionally, TIM-4 has been shown to be a receptor for phosphatidylserine and may play a role in the engulfment of apoptotic cells.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was generated by immunizing a female Lewis rat with Tim-4-Ig fusion protein.

Clone: 5G3

Isotype: IgG1 kappa

Application Details

Application Notes: The original version of this antibody (rat IgG1) specifically bound murine Tim-4 protein, as shown in ELISA and FC assays. The antibody was also used to immunoprecipitate Tim-4 protein from lysates of CHO-Tim-4 as part of WB assays (Rodriguez-Manzanet et al., 2008, PMID: 18354194). This antibody can block apoptotic body (AB) recognition by CD11b⁺ peritoneal macrophages and partially inhibit AB uptake by peritoneal B-1 cells (Rodriguez-Manzanet et al., 2010, PMID: 20368430). treatment with this antibody alone did not significantly affect tumor burden or survival, but it enhanced the protective effect of anti-PD-1 blockade. The combined treatment resulted in an increase in total CD8⁺ and CD8⁺ CD39⁺ T cells in the peritoneal cavity. RNA sequencing of sorted Tim-4⁺ macrophages revealed genes differentially expressed between animals treated with anti-PD-1 alone and those treated with both anti-PD-1 and this antibody, indicating that it alters the gene expression profile of Tim-4⁺ macrophages. Notably, it reduced the expression of genes associated with immunosuppression (Ptgs2, Tgfb2, and Tdo2) while increasing the expression of genes associated with macrophage activation (Stab1 and Mrc1). This antibody was used as part of single-cell RNA sequencing and T-cell receptor sequencing analyses. This antibody was used for FC staining in the form of flow sorting, co-culture experiments, and flow cytometric analysis to investigate various aspects of Tim-4 function, including its role in the sequestration of cytotoxic CD8⁺ T cells, the impairment of CD8⁺ T cell proliferation by cavity-resident macrophages, and the assessment of Tim-4-mediated suppression of CD8⁺ T cells both in vitro and in vivo. For example, it was used for FC staining in the form of annexin V staining to detect phosphatidylserine (PS) exposure on the surface of CD8⁺ T cells (Chow et al., 2021, PMID: 34115989).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for TIMD4

Target: TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))

Alternative Name: Tim-4 (TIMD4 Products)

Background: TIMD4, SMUCKLER, SMUCKLER, TIM4, Spleen, mucin-containing, knockout of lymphotoxin protein, T-cell immunoglobulin and mucin domain containing 4, T-cell immunoglobulin and mucin domain-containing protein 4, T-cell immunoglobulin mucin receptor 4, T-cell membrane protein 4, TIM-4, TIMD-4

UniProt: Q6U7R4

Pathways: Cancer Immune Checkpoints